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Product Info Summary
| SKU: | EKC1803 |
|---|---|
| Size: | 1 kit, containing one 96-well plate and all necessary reagents |
| Reactive Species: | Human, Mouse |
| Application: | ELISA |
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Product info
Product Name
TRA-2 alpha Colorimetric Cell-Based ELISA
SKU/Catalog Number
EKC1803
Size
1 kit, containing one 96-well plate and all necessary reagents
Description
The TRA-2 alpha Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRA-2 alpha protein expression profile in cells. The kit can be used for measuring the relative amounts of TRA-2 alpha in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRA-2 alpha.
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
TRA-2 alpha Colorimetric Cell-Based ELISA (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1803)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1803 is reactive to TRA2A in Human, Mouse samples
Application Guarantee
EKC1803 is guaranteed for ELISA in Human, Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 1 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-TRA2A Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 6ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 6ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 6ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
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Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from HuvEc cells
Protein Target Info & Infographic
Gene/Protein Information For TRA2A (Source: Uniprot.Org, NCBI)
Gene Name
TRA2A
Full Name
Transformer-2 protein homolog alpha
Weight
32.689kDa
Superfamily
splicing factor SR family
Alternative Names
HSU53209; htra-2 alpha; htra-2-alpha; putative MAPK activating protein PM24; TRA-2 alpha; tra2a; Tra2alpha; TRA2-alpha; transformer 2 alpha homolog (Drosophila); transformer-2 alpha; Transformer-2 protein homolog A; transformer-2 protein homolog alpha TRA2A AWMS1, HSU53209 transformer 2 alpha homolog transformer-2 protein homolog alpha|TRA-2 alpha|TRA2-alpha|Tra2alpha|htra-2 alpha|putative MAPK activating protein PM24|transformer-2 alpha|transformer-2 protein homolog A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TRA2A, check out the TRA2A Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TRA2A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For TRA-2 alpha Colorimetric Cell-Based ELISA (EKC1803)
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for TRA-2 alpha Colorimetric Cell-Based ELISA
Question
I am interested in using your EKC1803 TRA-2 alpha Colorimetric Cell-Based ELISA but I need to know if you have some information about its functionality in FACS or as stimulating antibody. Also I would like to ask if you know the epitope in TCRalpha that is recognized by this EKC1803 antibody.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
Unfortunately, this EKC1803 antibody has not yet been tested in these applications about its functionality in FACS or as stimulating antibody. All our tested applications are specified in our datasheets. For this antibody it is polyclonal to the whole recombinant protein and it recognizes many epitopes on the protein. If you want more specificity on the epitope, try a monoclonal antibody.
Boster Scientific Support
Answered: 2020-03-18
Question
Could you please tell me the immunogen was used to generate this TRA-2 alpha Colorimetric Cell-Based ELISA, because we want to use it with other species aside from human and we need to know its conservation as well as its chance of cross-reacting with other similar AP-2 family members.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The exact immunogen of this TRA-2 alpha Colorimetric Cell-Based ELISA is consider as proprietary, but we at least tell you that it is within the final 30 amino acids of the C terminus of the human protein. Please check this Uniprot accession P05549 (http://www.uniprot.org/uniprot/P05549).
Boster Scientific Support
Answered: 2019-11-12
